|
|
|
|
|
| CHAUD-NETTO,J.. |
| Malformed antennae of Apis mellifera light ocelli drones were drawn, dissected and mounted permanently on slides containing Canada balsam, in order to count the olfactory discs present in each segment, in comparison with the number of those structures in normal antennae of their brothers. Some drones presented morphological abnormalities in a single segment of the right or left antenna, but others had two or more malformed segments in a same antenna. Drones with malformations in both antennae were also observed. The 4th and 5th flagellum segments were the most frequently affected. In a low number of cases the frequency of olfactory discs in malformed segments did not differ from that one recorded for normal segments. However, in most cases studied, the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Apidae; Apis mellifera; Drones; Antennal malformations; Olfactory discs. |
| Ano: 2000 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0034-71082000000100015 |
| |
|
|
| COSTA,H.; TALORA,D. C.; PALMA,M.S.; CHAUD-NETTO,J.. |
| The effect of pheromones and their chemical analogues in honeybee alarm behaviors was studied in observation boxes. Defensive behaviors, as follows: a) attraction to scent source, b) elevation of wings in "V", c) abdomen elevation, d) abdomen elevation and pumping and e) first leg pair elevation had been temporarily registered when the following compounds were presented: isoamyl alcohol, octyl alcohol, benzyl alcohol, n-butyl acetate, n-octyl acetate, isopentyl acetate, benzyl acetate and 2-heptanone. The results were as follows: 1. the bees elicited some characteristic behaviors when chemical alarm messages were presented, 2. agression (stinging) was not completed with any compound tested, probably because there was not a target (visual stimulus), 3. in... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Apis mellifera; Defensive behavior; Alarm behaviors; Pheromones; Hybrid honey bees; Chemical communication. |
| Ano: 1996 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-79301996000100005 |
| |
|
| |
|
|
| LIMA,P. R. M. DE; BROCHETTO-BRAGA,M. R.; CHAUD-NETTO,J.. |
| Some properties of a Africanized honeybee venom proteases were determined by enzymatic assays in solution, electrophoresis in SDS-PAGE, and gel filtration. Bee venom extracts were obtained by reservoir disruption, selective dialysis (cut off 12 kDa) to eliminate small components, such as the protease inhibitor present in the venom, and then fractionation of the dialyzed extract by gel filtration on a Sephadex G-100 column. The optimal conditions for the caseinolytic assays were pH 9.5, 2-hour digestion at 37 °C, and 1% casein concentration. The proteolytic activity was also determined by electrophoresis in SDS-PAGE with co-polymerized gelatin with three major bands of 66.0, 41.6, and 25.1 kDa. A principal serine-protease-like mechanism was revealed in the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Venom; Africanized bee; Apis mellifera; Proteolytic activity; Gel filtration chromatography. |
| Ano: 2000 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-79302000000100004 |
| |
|
|
|
